You can use native python module hashlib which implements MD5 algorithm:
>>> import hashlib
>>> import os
>>> m = hashlib.md5()
>>> for root, dirs, files in os.walk(path):
for file_read in files:
full_path = os.path.join(root, file_read)
for line in open(full_path).readlines():
m.update(line)
>>> m.digest()
'pQ\x1b\xb9oC\x9bl\xea\xbf\x1d\xda\x16\xfe8\xcf'
You can save this result in a file or a variable, and compare it to the result of the next run. This will detect changes in any files, in any sub-directory.
This does not take into account file permission changes; if you need to monitor these change as well, this could be addressed via appending a string representing the permissions (accessible via os.stat for instance, attributes depend on your system) to the mvariable.